Olivamine Ingredients: Amino Acids

Glycine, L-taurine, N-acetyl-L-cysteine and L-proline

There are four amino acids found in Olivamine¹⁰. Glycine protects ATP-depleted cells by low affinity interactions with multimeric channel proteins. Glycine provided during ATP depletion blocked the development of membranous pores completely.³ The relationship between necrosis and an extra-cellular depletion of ATP makes its protection and restoration imperative during the pre-lethal stages of necrosis (non-programmed cell death) or early necrosis.

L-taurine can act as a direct antioxidant that scavenges or quenches oxygen free radicals intracellularly to block ROS mediated cell death. The beneficial effects of the ROS-scavenging capacity of L-taurine include attenuation of lipid peroxidation, reduction of membrane permeability, and inhibition of intracellular oxidation in different cells.⁴

N-acetyl-L-cysteine (NAC) is an antioxidant particularly against hydrogen peroxide. The hypothesis that NAC-induced free radical-signaling delays G0/G1 cells progression to S phase by regulating the cell cycle regulatory protein cyclin D1 and the free radical-scavenging enzyme manganese superoxide dismutase (MnSOD) has been investigated. Treatment with NAC resulted in increased cellular glutathione levels indicating a shift to a more reducing environment. These results support the hypothesis that cellular redox environment regulates cellular proliferation via regulating cell cycle regulatory protein levels.⁵

In studies done in vivo and in vitro, L-proline was found to be the only amino acid that was involved in the stimulation of DNA synthesis.⁶ Further, epidermal growth factor (EGF) elicited no response without the addition of L-proline.  L-Proline deficient media such as Leibovitz’s L-15, Eagle’s minimal essential, and Dulebecco’s modified minimal essential did not induce DNA synthesis. However, using media such as Williams E, McCoy’s 5A and Ham’s F-12, which are rich in L-proline, there was DNA synthesis and marked proliferation.⁷